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KB001381: 我可以使用流路和限制峰面积来计算损耗效率吗?
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我可以使用流路和限制峰面积来计算损耗效率吗?
不可以 – 流速的改变以及蛋白质对UV的响应变化都使得使用峰面积/吸光度变得不准确. 一般来说, 人们可以重叠色谱图以察看运行之间的重现性，并保证流路峰和限制峰不变. 但是，与峰面积或峰高的直接关系计算除去效率是不准确的.
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