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What's New in Version A.04.00
 Learn more about how Version A.04.00 incorporated enhancements recommended by actual DRS customers to improve usability.
- Quantitation of AMDIS deconvoluted result in MSD ChemStation QEdit
- Summary reports of both MSD and AMDIS deconvoluted result for efficient review
- Requires Agilent MSD Productivity ChemStation Rev E.02.00 and Agilent NIST 2005 MS Library
- One of the Agilent RTL Libraries is highly recommended
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What is DRS?
Agilent Deconvolution Reporting Software (MSD-DRS) is an application for target compound analyses that combines results from the Agilent MSD Productivity ChemStation, the NIST Automated Mass spectral Deconvolution and Identification Software (AMDIS), and the NIST 2005 Mass Spectral Search Program (NIST) into one easy-to-read report.
DRS (G1716AA) works with any Agilent RTL Database Library. DRS can work with full-scan or SIM data.
Benefits:
- Significant time and cost savings
- Automated and fast; as little as one minute per sample of processing time
- Deconvolution works especially well with dirty matrices
- Greater accuracy
- More thorough than standard quantification and library search methods
- Finds additional targets not found by the MSD Productivity ChemStation or the analyst
- Easy-to-use
- Can do screening and quantitation in one run
- No need to learn a separate deconvolution software
What is Deconvolution?
From NIST AMDIS: “The term is used here in the broad sense of extracting one signal from a complex mixture. The treatment of noise, the correction for base line drift, and the extraction of closely co-eluting peaks from one another is all part of the deconvolution process.”
The deconvolution can be explained using the following simplified graph:
How does DRS work?
MSD-DRS runs from the DRS pull-down menu in MSD Productivity ChemStation Enhanced mode data analysis. It can also be set up to run automatically after data acquisition or in data reprocessing mode. Typical total processing time is one to two minutes, depending upon sample complexity and computer capability:
- The MSD Productivity ChemStation identifies targets by integrating extracted ions and comparing ion ratios to known ratios. Targets found are saved in a table for later use.
- AMDIS deconvolutes the ChemStation data file. These “cleaned” spectra (“components” as called in AMDIS) are compared against a user-constructed library. This comparison uses full spectra (or SIM data) and is retention time (RT) independent. Identified targets are saved in a table for later use and are also sent to NIST.
- NIST searches all spectra sent by AMDIS against the NIST >190,000 compound main library. This is also a full spectrum search, but now against a different library than used by AMDIS. The user can choose to skip the NIST search in processing SIM data.
- Final report generation combines results from the three techniques described above. Results are compared and sorted. A printed report can be generated together with a file in “html” format.
The following graph shows the DRS processes a complex total ion chromatogram (TIC) with over 370 peaks into an easy-to-read report in about one minute.
With the Rev. A.04 you can import and quantitate the AMDIS deconvoluted result in MSD ChemStation QEdit…
DRS reports of both MSD and AMDIS deconvoluted result for efficient review
DRS Literature
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