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- Superior nebulization delivers more ions to the mass spectrometer
- High drying capacity improves performance
- Orthogonal nebulization simplified operation and reduces maintenance
- Dual nebulizers maximize mass accuracy
- Multiple ionization techniques to serve more application
- more...
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| Multimode source |
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- The only ion source capable of simultaneously generating ions by electrospray (ESI) and atmospheric pressure chemical ionization (APCI)
- Identifies very diverse compounds in a single run with spectrum-to-spectrum polarity switching
- Operates in ESI-only or APCI-only mode
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Electrospray (ESI) |
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- Multiple charging and relatively low operating temperatures allow for analysis of biological macro-molecules such as proteins and peptides, as well as for smaller, more polar molecules.
- Concentration-sensitive technique: more concentrated samples improve sensitivity
- Available for Standard-flow-rate electrospray, Capillary electrospray, and nanoelectrospray
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| Atmospheric pressure chemical ionization (APCI) |
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- Popular complement to electrospray, generates primarily singly charged ions and operates at higher temperatures for wide range of less polar and non-polar molecules
- Mass-flow sensitive technique: more sample molecules improve performance
- Works better with larger columns and higher LC flow rates
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| HPLC-Chip nanoelectrospray |
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- Reusable HPLC-Chips seamlessly integrate the sample enrichment and separation nanocolumns, tubing, connections, and spray needle of a traditional nanoelectrospray LC/MS system into a biocompatible polymer chip
- Narrower, better-defined peaks due to elimination of post-column dead volumes
- Concentration-dependent technique: HPLC-Chip can dramatically improve sensitivity
- Sealed, robotic interface for fully automated handling
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| Atmospheric pressure photoionization (APPI) |
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- For analysis of compounds that ionize poorly by ESI and APCI
- Syagen Technology ensures that RF-resonated krypton lamp emits photons at energy levels high enough to ionize many classes of compounds with minimal ionization of air and common HPLC solvents
- Easily determines molecular weights due to minimal fragmentation
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| Atmospheric-pressure MALDI (AP/MALDI) |
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- Provides fast, easy and ultrasensitive analysis of 2D gel-spot digests and samples that do not need online separation with integrated pulsed dynamic focusing
- Excellent solution for high throughput screening, expression profiling studies, and protein identification and confirmation using peptide mass fingerprinting
- Available for purchase from MassTech
- Application Notes
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Application of Ionization Techniques |
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- No ionization technique is suitable for all applications, but Agilent offers you a wide range of choices
- View application table
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