Analysis
of beta-blocking drugs on Agilent 1200 Series LC systems using UV, fluorescence
and mass spectrometry
Beta-blocking drugs are widely used in the treatment of hypertension
and glaucoma. The determination of these drugs in biological fluids is nowadays
done by fluorescence or mass spectrometry in combination with standard bore
or narrow bore columns1-3. With fluorescence detection beta blocking drugs
are analyzed in the low ng range. The disadvantage is that not all beta-blocking
drugs fluoresce. These compounds can be analyzed using UV detection. The
disadvantage of using UV detection is that it is typically less sensitive
than fluorescence detection. Recently mass spectrometry is also being used
to identify betablocking drugs and their metabolites in the low pg range.
Detailed Application Notes
Overview
Agilent 1200 Series