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Ordering details - Off-Line 2D Nano LC-MS 

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Recommended System Configuration

1st dimension LC system - 1260 Infinity Micro-fraction Collection System

Agilent 1260 Infinity Capillary Pump with micro vacuum degasser

G1382A

Agilent 1200 Series 2-position/10-port micro valve

G1163A

Agilent 1260 Infinity High Performance Micro Autosampler

G1377A

Agilent 1260 Infinity Diode-array Detector

G1315B014

Agilent 1260 Infinity Micro Fraction Collector
Includes the special micro fraction collection capillaries, 25 µm id (G1364-87304), 50-µm id (G1364-87305), and 100-µm id (G1364-87306), as well as the 1.12 m length, 50-µm id capillary (5065-9906) to connect the capillary pump to the column in the thermostatted column compartment

G1364D

2nd dimension LC system

Agilent 1260 Infinity Nanoflow LC System for MS

G2229A

Agilent 6500 Series Accurate Mass Q-TOF LC/MS

G6530 or G6540

Orthogonal nanoelectrospray source

G1982B

Nanoflow LC start-up kit

G2228-68700

Agilent 1200 Infinity Series Column Organizer

G1383A

Spectrum Mill MS Proteomics Workbench

G2721A

Thermostat for Agilent 1260 Infinity Autosampler, Well-plate Sampler and Fraction Collector

G1330B


Recommended Chromatographic Columns
Ion Exchange column(s) for the 1st dimension of 2D LC
ZORBAX BioSCX Series II, 300 µm × 35 mm, 3.5 µm; strong cation exchanger 5065-9912
ZORBAX BioSCX Series II, 0.8 mm × 50 mm, 3.5 µm; strong cation exchanger 5065-9942
Reversed-phase column for peptide enrichment before 2nd dimension of 2D LC
ZORBAX 300SB-C18, 300 µm × 5 mm, 5 µm (5/pk) (included in G2228A Nanoflow LC start-up kit) 5065-9913
Reversed-phase columns for 2nd dimension of 2D LC:
ZORBAX 300SB-C18, 75 µm × 150 mm, 3.5 µm (included in G2228A Nanoflow LC start-up kit) 5065-9911

 

 
 
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