Multidimensional LC for Separation of Protein Digests
Multidimensional liquid chromatography (MDLC) with orthogonal separation mechanisms offers significant benefits for the separation of complex protein digests from biological samples. MDLC can be easily automated and offers high reproducibility, since it is performed in liquid phase. In addition it works well for hydrophobic, acidic, basic, very small, very large and low abundance proteins that may be difficult to analyze using more traditional separation techniques. Multidimensional LC can be implemented in a number of ways and can be tailored to the requirements of particular sample types.
 On-line 2D Nano LC/MS
On-line 2D Nano LC/MS is well suited for digests from gel spots or bands which need further separation and for subcellular proteome fractions of higher complexity. It is also useful for identifying biomarkers in pre-enriched biological fluids like blood, spinal fluid or urine. Finally, it is the best choice for further separation of affinity-purified post-translationally modified (PTM) peptides or protein complexes for functional proteomics research.
Off-line 2D Nano LC/MS
Off-line 2D Nano LC/MS is well suited for extremely complex proteome samples such as whole cell lysates or tissues. It can also be applied to purification and analysis of low abundance proteins or biomarkers from highly complex biological matrices
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