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Recommended System Configuration |
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Agilent 1260 Infinity Nanoflow LC system for MS |
G2229A |
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Agilent 1200 Series 2-position/10-port micro valve |
G1163A |
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Agilent 1260 Infinity Capillary Pump with micro vacuum degasser |
G1382A |
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Agilent 6500 Series Accurate Mass Q-TOF LC/MS |
G6530 or G6540 |
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Orthogonal nanoelectrospray source |
G1982B |
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Nanoflow LC start-up kit |
G222868700 |
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Agilent 1200 Infinity Series Column Organizer |
G1383A |
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Spectrum Mill MS Proteomics Workbench |
G2721A |
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Thermostat for Agilent 1260 Infinity Autosampler, Well-plate Sampler and Fraction Collector |
G1330B |
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Recommended Chromatographic Columns |
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Ion Exchange column(s) for the 1st dimension of 2D LC |
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ZORBAX BioSCX Series II, 300 µm × 35 mm, 3.5 µm; strong cation exchanger |
5065-9912 |
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ZORBAX BioSCX Series II, 0.8 mm × 50 mm, 3.5 µm; strong cation exchanger |
5065-9942 |
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Reversed-phase column for peptide enrichment before 2nd dimension of 2D LC |
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ZORBAX 300SB-C18, 300 µm × 5 mm, 5 µm (5/pk) (included in G2228A Nanoflow LC start-up kit) |
5065-9913 |
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Reversed-phase columns for 2nd dimension of 2D LC: |
… |
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ZORBAX 300SB-C18, 75 µm × 150 mm, 3.5 µm (included in G2228A Nanoflow LC start-up kit) |
5065-9911 |
 Back to On-line 2D Nano LC/MS
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