RNA Sample QC derived from laser microdissected tissue samples obtained by the PALM® MicroBeam System
 Gene expression profiling of material isolated by microdissection has become a very popular method for analyzing cellular behavior in a micro scale and is used in a wide range of research and clinical applications. Laser-assisted microdissection allows isolation of RNA from specific cell populations out of their surrounding tissue environment. Usually, the sample yield that can be obtained by this method is too low for quality control of RNA using a standard technique. On the other hand, extraction of high quality RNA is crucial for all subsequent steps and the success of the overall experiment. Since the introduction of the Agilent RNA 6000 Pico LabChip® kit, it is possible for the first time to analyze total RNA samples in concentrations down to 200 pg/µl, which is in the range of the sample concentration one can expect from microdissected tissue or cells. In this example, RNA samples that were obtained by laser microdissection and pressure catapulting on the PALM® MicroBeam System from tissue sections were analyzed.
To read more about this application click here.
To learn more about the PALM MicroBeam System click here.
 Return to RNA Solutions
|