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Guarantee high quality RNA for microarray sample preparation
RNA quality is essential for confidence in gene expression analysis using microarray technology. Agarose gel electrophoresis can be used to monitor the quality of RNA preparations, however many researchers forgo this RNA check because of the lengthy protocols and the large amount of sample required.

Agilent offers RNA assays that allow characterization of total or mRNA samples and determination of their concentrations.

  • High sensitivity with small sample volume
    As little as 5 ng/µl RNA is required per analysis.
    This saves you sample for any downstream experiments.
  • Catch RNA degradation right away
    RNAse degradation of total RNA is easily detected by a shift in the RNA size distribution towards smaller fragments and a decrease in fluorescence signal of ribosomal peaks
  • Automated detection of ribosomal RNA contamination
    The Agilent 2100 bioanalyzer software integrates the messenger RNA smear and calculates the percentage of ribosomal RNA contamination.

    The figure below shows the most important steps in the workflow of preparing cRNA samples for microarray experiments that can be monitored on the Agilent 2100 bioanalyzer.

More information about Agilent's microarray solutions


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