Ensure successful protein purification along each step!
Purifying a protein from crude extracts or whole cell lysates is tedious and involves many steps in which a specific protein can be lost. Tracking the presence of the protein with SDS-PAGE is often ignored because it makes the purification procedure significantly more time consuming and laborious. The Agilent 2100 bioanalyzer automatically incorporates many manual steps. Combined with the Protein 200 Plus Chip assay it can be used to analyze cell lysates, column fractions and purified proteins in under 30 minutes.
- Be in control
Digital operation enables precise observation of your purification procedure
- Save time
Press 'START' and see your first results after just 7 minutes. Subsequent results at 2 minute intervals.
- No manual staining and destaining steps required
During the run, samples are automatically stained and destained. This avoids the manual steps used in conventional SDS-PAGE.
- Direct comparison of samples
The Agilent 2100 bioanalyzer software allows overlaying of sample data for instant comparison of different samples. With this you can easily detect the expression of a recombinant protein when comparing cell lysates.
- No more gels to dry
The data, together with the sample information, assay protocols and additional comments, is automatically stored in digital format and can be easily retrieved and shared. This eliminates the need to dry gels and to store them in a lab book.
Related Application Notes
- "Optimization of protein purification using the Agilent 2100 bioanalyzer" (5988-4022EN)
- "Comparison of different methods for purification analysis of a green fluorescent Strep-tag fusion protein" (5988-5025EN)
- "Protein purification and characterization using the 1100 Series purification system and the 2100 bioanalyzer" (5988-8630EN)
- "Using the Agilent 2100 bioanalyzer for analysis of His-tag removal from recombinant proteins" (5988-8144EN)
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