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2100 Bioanalyzer - Protein Solutions 

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Agilent Protein 250 High Sensitivity kit - Silver stained PA-gel – 2100 Pseudo gel view 
High sensitivity protein detection at pg levels 
In combination with the High Sensitivity Protein 250 kit, the Agilent 2100 Bioanalyzer detects proteins down to 1 pg/μL on chip, which is superior to silver stain SDS-PAGE. It provides quantification over a dynamic range of up to 4 orders of magnitude, is highly reproducible, which is particularly important for the analysis and quality control of proteins. Traditionally, silver stain methods were used in electrophoresis for protein detection at the low picogram range.
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Features
  • Highest sensitivity – silver stain sensitivity down to 1 pg/µL of labeled protein on chip
  • Wide linear dynamic range – quantitative range with 4 orders of magnitude. Detect a 0.05% impurity while visualizing intense main product with reliable quantification
  • Fast results – Labeling reaction and 10 samples per chip done in half the time required for typical silver staining methods
  • Quality, quantity and purity in one run – results include quantification for each individual protein, providing purity measurements with 4 lorders of magnitufe of linear dynamic range
  • Ready-to-use - standardized assays and pre-packaged reagent kits

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