Probe coverage characteristics on Agilent human 244A and 105A microarray
Typical probe coverage characteristics on Agilent 244A and 105A CGH microarrays as illustrated using UCSC hg18 Human Genome Browser, Feb 2006 (NCBI build 36) of chromosome 17. At the top of each view, each short vertical bar represents a single probe on Agilent Human Genome CGH microarray 244A (blue) or 105A (red). Annotations below the probes indicate the location mapping of UCSC known genes (blue or black) and Sanger microRNAs (red).
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A 3.0 MB window of 17q24-25 shows comprehensive coverage over coding region and non-coding regions.
A 3.0 MB window of 17q11-12 depicts multiple-probe coverage evenly distributed across long (ACCN1) as well as short (NLE1) transcripts.
A 1.0 MB region of 17q11.2 illustrates microRNA representations (hsa-mir-193a, hsa-mir-365-2).
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Enhanced Precision and Novel Detection on the Agilent human 244A microarray
Genomic variations include both gains and losses of chromosomal regions that often vary extensively in their sizes. The enhanced detection resolution enabled through the high-density microarrays delivers the highest precision in mapping chromosome breakpoints and identification of micro-variations that are not detected by other platforms.
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CGH Analytics chromosome view. |
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Zoomed-in gene view of chromosome 3 in the human colon carcinoma cell line HT29. |