Gene Expression Quantification
The DNA 1000 Chip kit can be used in conjunction with specific (RT)-PCR kits or assays to quantify expression of specific genes in cancer diagnostics and cancer research as well as other application areas. If properly designed, these assays can provide quantitative and precise results over a wide dynamic range. This multiplex approach can analyze many more genes per reaction than techniques such as real-time RT-PCR and provides accuracy, convenience, and many other advantages over standard gel electrophoresis. See an application example for 19-plex multiplex PCR.Our partners QIAGEN and Maxim Biotech have specialized products for multiplex PCR applications.
DNA Quantification and QC for Next Generation Sequencing
The advent of NGS has dramatically changed the genetic landscape by significantly increasing experimental speed of whole genome or transcriptome sequencing projects. The Agilent 2100 Bioanalyzer can be used for monitoring critical steps in the workflow of NGS experiments including DNA fragmentation, target enrichment, and DNA library amplification. Many NGS sample preparation protocols require DNA library amplification by PCR which can often introduce amplification bias. The improved detection sensitivity of the High Sensitivity DNA kit significantly lowers the number of required PCR cycles, reducing amplification artifacts and improving the quality of sequencing data.
Detection of Genetically Modified GMOs
The DNA 1000 Chip kit can be used to detect and quantify GMOs. Methods using the DNA 1000 LabChip kit have been developed with the advantage of multiplex several different GMOs in one test.
"Detecting genetically modified organisms with the Agilent 2100 bioanalyzer" ( 107 KB)
"Analysis of genetically modified soya using the Agilent 2100 bioanalyzer" (80 KB)
Additional DNA Sizing and Quantification Applications
The DNA Chip kits can be used for a multitude of other applications including restriction digest fragment analysis and template quantification prior to cloning or sequencing experiments.
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