Agilent is committed to bringing scientists robust research tools that will accelerate their research.
Case in point, Agilent is the first open systems microarray-based gene expression solutions provider to
offer a whole human genome microarray designed for the masses. Leveraging Agilent’s 1 x 3" (25mm x 75mm)
glass slide, 44K feature microarray format, researchers can now run high-quality, commercially-printed whole
genome microarrays in most lab environments leveraging existing set-ups and scanning equipment.
| 1. Feature Layout |
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Agilent's SurePrint 60-mer in situ manufacturing process enables true flexibility in microarray layout and format for generation of the Whole Human Genome microarray. |
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| 2. Image Quality |
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Demonstration of cross-performance of Agilent's 60-mer oligo microarrays shows unparalleled results.
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Shown here are experiments where Agilent's 22K (single density) oligo microarrays were compared to Agilent's new 44K (double density) format oligo microarrays. The testing proves that both formats deliver robust linearity and sensitivity across a wide dynamic range, down to ratios measured with 0.5 to 1.5 copies per cell.
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3. 60-mer Probe Design
 Creation of whole human genome microarray,
Expert. Rev. Proteomics 1 (1): 19-28, 2004
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A key benefit to Agilent's flexible inkjet manufacturing process is that enables iterative design of the Whole Human Genome microarray's probes.
The graph depicts the selection of consensus regions and probes to represent all transcripts within a GeneBin. The transcripts are used to determine the location of consensus regions (Numbered 1, 2, and 3).
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The plot presents clustering data that demonstrates that probes for consensus regions 2 and 3 exhibit similar behavior, while probes for consensus region 1 exhibit different behavior, and thus represent, in this case, a real alternative 3' end of transcript A.
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Histogram showing the number of probes on the Whole Human Genome related to their distance from the 3' end of the target transcript. Degraded RNAs generally are detected more effectively with 3' biased probes
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| 4. Reproducible Data |
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Low noise and excellent microarray-to-microarray format reproducibility leads to more consistent data.
Shown here are results of experiments that determined the data correlation between Agilent's 22K Human Oligo Microarrays and the higher-density 44K Whole Human Microarray format.
Complex human targets were run on each type of microarray and subsequent analysis of the combined GREEN signals and RED signals yield tight single color data correlation from the 22K to 44K microarray formats. Further analysis of the mean log BLUE ratios of the complex human targets show a favorable R2 value of 0.9797 between the two microarray formats.
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