Processing of the Oligo aCGH microarrays can require additional reagents and hardware not included in the kit. Tables below list those items.
Reagents
|
Processing Step |
Product Name |
Use |
|
Sample Labeling |
Genomic DNA 96-Well Purification Module |
Used for ULS-labeled genomic DNA purification. Contents include 1 96-well purification plate, 1 wash plate, and 1 collection plate. |
|
Genomic DNA Enyzmatic Labeling Kit |
Genomic DNA labeling kit validated and optimized for DNA isolated from FFPE, cells, blood, and tissue. Contains sufficient two-color labeling reaction reagents for 5 microarray slides of the formats 1-pack, 2-pack, 4-pack or 8-pack. Contains sufficient purification columns for processing 5 – 1-pack microarrays. Additional columns and tubes should be ordered separately when using multi-pack microarrays (5190-0418). |
|
Genomic DNA Purification Module |
Used for ULS-labeled genomic DNA purification. Contents include 1 96-well purification plate, 1 wash plate, and 1 collection plate. |
|
Genomic DNA ULS Labeling Kit |
Genomic DNA labeling kit validated and optimized for DNA isolated from FFPE, cells, blood, and tissue. Contains sufficient two-color labeling reaction reagents for 5 microarray slides of the formats 1-pack, 2-pack, 4-pack or 8-pack. Contains sufficient purification columns for processing 5 – 1-pack microarrays. Additional columns and tubes should be ordered separately when using multi-pack microarrays (5190-0418). |
|
Genomic DNA High-Throughput ULS Labeling Kit |
Genomic DNA labeling kit validated and optimized for DNA isolated from FFPE, cells, blood, and tissue. Contains sufficient two-color labeling reaction reagents for 16 - 1-pack, 24 - 2-pack, 48 - 4-pack, and 96 - 8-pack microarrays. Customers should purchase either the Genomic DNA Purification Module (5190-0418) or the Genomic DNA 96-well Purification Module (5190-0451) to purify the labeled genomic DNA. |
|
Sample Hybridization |
Oligo aCGH/ChIP-on-chip Hybridization Kit |
Contains 2X Hi-RPM Hybridization Solution and 10X Blocking Agent for 25 slides. Customer must purchase the following to prepare hybridization solution: Human Cot-1 DNA (Invitrogen part numbers 15279-011 or 15279-101 and Kreatech part number EA-020) or Mouse Cot-1 DNA (Invitrogen part number 18440-016) or Rat Hybloc (Applied Genetics part number RHB). DNase/RNase-Free Distilled Water (Invitrogen part number 10977-015). |
|
Oligo aCGH/ChIP-on-chip Hyb Kit, Large Volume |
Contains 2X Hi-RPM Hybridization Solution and 10X CGH Blocking Agent for 100 slides. Customer must purchase the following to prepare hybridization solution: Human Cot-1 DNA (Invitrogen part numbers 15279-011 or 15279-101 and Kreatech part number EA-020) or Mouse Cot-1 DNA (Invitrogen part number 18440-016) or Rat Hybloc (Applied Genetics part number RHB). DNase/RNase-Free Distilled Water (Invitrogen part number 10977-015). |
|
Oligo aCGH Prehybridization Buffer (for Tecan HS Pro Station) |
For use in Tecan processing protocols only. Sufficient prehybridization buffer for approximately 120 slides when processed on the Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. |
|
Microarray Wash |
Oligo aCGH/ChIP-on-chip Wash Buffer Kit |
This kit contains Oligo aCGH Wash Buffer 1 (4L, part number 5188-5221) and Oligo Wash Buffer 2 (4L, part number 5188-5222).
This kit is available in two configurations:
Option 1 for manual processing contains 2 containers of Wash Buffer 1 and 1 container of Wash Buffer 2. The kit is sufficient for processing up to 40 oligo aCGH slides.
Option 2 for Tecan processing contains 1 container of Wash Buffer 1 and 2 containers of Wash Buffer 2. The kit is sufficient for processing up to 120 oligo aCGH slides. |
|
Stabilization and Drying Solution |
Contains reagent that prevents ozone-induced degradation of cyanine dyes during processing and scanning of Agilent oligo-based microarrays.
Sufficient reagent for up to 25 oligo aCGH slides. Customer must purchase the following to use this product with oligo aCGH arrays: Acetonitrile (Sigma-Aldrich 271004-1L). |
|
Microarray Wash Buffer Additive (for Tecan HS Pro Station) |
For use in Tecan processing protocols only. Solution is used to prepare Conditioning Solution and Wash Buffer 2 for processing oligo aCGH arrays on the Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. Sufficient for approximately 120 slides. |
Hardware
|
Hardware Type |
Product Name |
Description |
|
Sample QC |
2100 Bioanalyzer System (optional) |
Includes 2100 Bioanalyzer with HP/Compaq desktop PC, printer, 2100 expert software, vortex mixer, accessories, without cartridge and license. |
|
RNA 6000 Nano LabChip Kit (optional) |
Includes reagents, supplies, 25 LabChips, 2 cleaning chips, syringe, and reagent kit guide. |
|
Hybridization Chambers & Accessories |
Hybridization Chamber - SureHyb |
Includes chamber base, chamber top, clamp, chamber thumbscrew, plastic tweezers and a microarray hybridization user guide. |
|
Hybridization Backing Kit |
Includes gasket slides for the single-plex microarrays in kit sizes of 5, 20, or 100 gaskets |
|
Hybridization Oven & Accessories |
Hybridization Oven |
Equipped with a removal rotator rack, and designed with user-settable rotator speeds and variable hybridization temperatures. |
|
Hybridization Chamber Rotator Rack |
Rotator rack for holding hybridization chambers during incubation in hybridization oven. |
|
DNA Microarray Scanner |
Scanner bundle |
Includes: DNA microarray scanner, 48-position carousel with cover, 2 slide holder kits (50 slide holders), crossover LAN cable, Feature Extraction and Analysis software, PC data system, installation and familiarization, and one-year service warranty and technical support. | |
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