Processing of the Gene Expression microarrays can require additional reagents and hardware not included in the kit. Tables below list those items.
Reagents
|
Processing Step |
Product Name |
Use |
|
Sample Preparation & Isolation |
Total RNA Isolation Mini Kit |
Contains reagents, mini-filtration columns, mini-isolation columns, and final nuclease-free collection tubes for 50 total RNA isolations from mammalian cells (5 x 105- 5 x 106), tissues (2.5 – 30 mg), yeast (107) and bacteria (105) |
|
Plant RNA Isolation Mini Kit |
Contains reagents, mini-filtration columns, mini-isolationc olumns, and final nuclease-free collection tubes for 50 total RNA isolations from a wide range of species and tissue types |
|
Sample Labeling & Amplification |
Fairplay III Labeling Kit |
For E. coli Microarray only.
Converts total RNA to fluorescently labeled cDNA using a two-step chemical coupling method. Includes random primers for use with prokaryotic samples |
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One Color
Quick Amp Labeling Kit, one-color
|
Contains Cyanine 3-CTP and enzyme kit to amplify and label 50-200 ng target mRNA species to complementary RNA (cRNA) for oligo microarrays. Kit is sufficient for 20 reactions. Quick Amp Labeling Kit with Cyanine 5-CTP is also available (Part Number 5190-0445).
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Dual Color
Quick Amp Labeling Kit, two-color
|
Contains Cyanine 3-, Cyanine 5-CTP, and reagents to amplify and label 50-200 ng target mRNA species to complementary RNA (cRNA) for oligo microarrays. Each vial of Cyanine CTP is sufficient for 10 reactions. Kit is sufficient for 20 reactions. Quick Amp Labeling Kit with Cyanine 5-CTP is also available (Part Number 5190-0445).
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Microarray Hybridization |
Gene Expression Hybridization Kit |
Contains 2X Gene Expression Hybridization Solution, 2X Hi-RPM Hybridization Buffer, 25X Fragmentation Buffer, and 10X Gene Expression Blocking Agent for at least 10 slides. |
|
Hi-RPM Gene Expression Hyb Kit, Large Volume |
Contains 2X Hi-RPM Gene Expression Hybridization Buffer, 25X Fragmentation Buffer, and 10X Gene Expression Blocking Agent for at least 100 slides. Components may be purchased separately. |
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One-Color RNA Spike-In Kit |
Contains dilution buffer and a pre-mixed cocktail in a predetermined ratio-generating concentration to serve as a positive microarray control. Kit sufficient for at least 200 microarray hybridizations. |
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Two-Color RNA Spike-In Kit |
Contains dilution buffer and two pre-mixed cocktails in predetermined ratio-generating concentrations that serve as positive microarray controls. Kit sufficient for at least 200 microarray hybridizations. |
|
Microarray Wash |
Gene Expression Wash Buffer Kit |
Contains two 5188-5325 (Gene Expression Wash Buffer 1, 4L each), one 5188-5326 (Gene Expression Wash Buffer 2, 4L) and Triton X-102 detergent additive. Kit contains sufficient wash buffer for up to 80 slides. Wash buffers may be purchased separately. |
|
Stabilization and Drying Solution |
Contains reagent that prevents ozone-induced degradation of cyanine dyes during processing and scanning of Agilent oligo-based microarrays. Sufficient stabilization and drying solution for up to 50 gene expression slides. Customer must purchase the following to use this product with oligo aCGH arrays: Acetonitrile (Sigma-Aldrich 271004-1L). |
Hardware
|
Hardware Type |
Product Name |
Description |
|
Sample QC |
2100 Bioanalyzer System
(optional) |
Includes 2100 Bioanalyzer with HP/Compaq desktop PC, printer, 2100 expert software, vortex mixer, accessories, without cartridge and license. |
|
RNA 6000 Nano LabChip Kit (optional) |
Includes reagents, supplies, 25 LabChips, 2 cleaning chips, 1 syringe, and reagent kit guide. |
|
Hybridization Chambers & Accessories |
Hybridization Chamber - SureHyb |
Includes chamber base, chamber top, clamp, chamber thumbscrew, plastic tweezers and a microarray hybridization user guide. |
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Hybridization Backing Kit |
Includes gasket slides for the x 44K microarrays in kit sizes of 5, 20, or 100 gaskets. |
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Hybridization Oven & Accessories |
Hybridization Oven |
Equipped with a removal rotator rack, and designed with user-settable rotator speeds and variable hybridization temperatures. |
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Hybridization Chamber Rotator Rack |
Rotator Rack for holding hybridization chambers during incubation in hybridization oven. |
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DNA Microarray Scanner |
Scanner bundle |
Includes: DNA microarray scanner, 48-position carousel with cover, 2 slide holder kits (50 slide holders), crossover LAN cable, Feature Extraction and Analysis software, PC data system, installation and familiarization, and one-year service warranty and technical support. | |
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