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One-Color RNA Spike-In Kit 

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Monitor for sensitivity and accuracy 
The Agilent One-Color RNA Spike-In Kit contains in vitro synthesized, polyadenylated transcripts. When hybridized onto a microarray, the user can use the expected versus observed log ratios to monitor a microarray workflow for linearity, sensitivity and accuracy. This kit is a required component of the Agilent One-Color Gene Expression platform.
Research use only.
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Features
  • Sample required per reaction is 100 - >500 ng total RNA
  • Each transcript was constructed by the cloning of a unique 55-mer sequence into the human adenovirus type 6 E1A 13S gene
  • Transcripts specifically anneal only to complementary microarray probes with minimal self- or cross-hybridization
  • Spike-In transcripts specifically anneal only to complementary control probes on Agilent’s microarrays, with minimal self- or cross-hybridization
  • Dynamic range is approximately 6 orders of magnitude
  • Contents include spike mix and dilution buffer
  • Kit should be stored initially at –80°C for up to one year from date of result. Diluted spike mixes can be stored at –20°C for up 2 months.

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