Showcase - Human Genome CGH Microarray 44B 

Learn more about the biological utility and performance capability of this new array-based Comparative Genomic Hybridization (aCGH) technology platform from the following publication.

Comparative Genomic Hybridization using Oligonucleotide Microarrays and Total Genomic DNA
Michael T. Barrett, Alicia Scheffer, Amir Ben-Dor, Nick Sampas, Doron Lipson, Robert Kincaid, Peter Tsang, Bo Curry, Kristin Baird, Paul S. Meltzer, Zohar Yakhini, Laurakay Bruhn, and Stephen Laderman.
Proc. Natl. Acad. Sci. USA 101 (51): 17765-17770 (2004).

Genome Coverage by Chromosome
Zoom	Each 60-mer oligonucleotide probe is represented by a blue dot. Average spatial resolution = 35kb.
Detection of varying copy numbers of the X chromosome The accuracy and detection capability of the system was clearly demonstrated with hybridizations of genomic DNA samples from a series of cell lines with variable copy number of the X chromosome using 46, XX DNA as a common reference.
Zoom	(A) Distributions and medians of log(2) ratio values of all X-chromosome oligonucleotide probes in five hybridizations on the Human Genome CGH Microarray 44A (G4410A): XY/XX (blue), XX/XX (green), XXX/XX (red), XXXX/XX (teal), and XXXXX/XX (purple).
Zoom	(B) Measured mean log(2) ratios of all X-chromosome probes (Y-axis) are plotted versus the corresponding theoretical log(2) ratios (X-axis).
Detection sensitivity and reproducibility. The ability to detect single copy loss is critical in identification of new tumor suppressor genes and microdeletions associated with genetic diseases. The detection sensitivity of the system was demonstrated in the following hybridization experiment using 46, XY and 46, XX genomic DNA samples.
A. Zoom B. Zoom	CGH Analytics view of Chromosome 1 (A) and Chromosome X (B) from two dye-flip pairs of XY/XX experiment (10 pt moving average).
C. Zoom	(C) Chromosome 1 plot of a self versus self experiment, demonstrating the baseline system noise of the platform.