HPLC Columns for Biotechnology 

The ZORBAX Oligo column is designed for the high resolution gradient separation and purification of oligonucleotides. Oligonucleotides from 3 - 48 bases have been purified using this column, while it is possible to separate oligonucleotides up to the 60 - 80 mer range. The Oligo column uses a mixed mode ion-exchange and reversed-phase bonded phase developed for oligonucleotides in order to effectively isolate them with high purity. Simple gradient mobile phases are used for oligomer separations with the possible addition of denaturants, such as urea, to prevent self-hybridization. The Oligo column will provide high resolution for samples from less than 20 micrograms up to 300 micrograms in a volume of up to 1 mL.