HPLC Columns for Biotechnology 

Ideal for the size separations of proteins and other biomolecules. The separation range is 4,000 - 900,000 for globular proteins when using a GF-250 and GF-450 column in series. The GF-250/450size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and unique zirconia modification of the silica to extend the pH range from 3 - 8. The GF-250/450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions. The result is a highly efficient, rugged and reproducible size exclusion column for separations of proteins with flow rates of up to 3 mL/min. These columns are compatible (typically 10% v/v or less) with organic modifiers and denaturants in the mobile phase to eliminate protein aggregation for proper size determination.