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Multiple Affinity Removal LC Column - Human 14 

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Multiple Affinity Removal LC Column - Human 14 
Simple method removes 14 abundant proteins 
In order to more easily isolate and identify proteins in human serum, the Agilent Human 14 Multiple Affinity Removal Column is designed to chromatographically remove fourteen interfering high-abundant proteins from human plasma samples. Removal of these abundant proteins improves the subsequent LC/MS and electrophoretic analysis of the serum sample by effectively expanding the dynamic range of the analysis. Column Specifications

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Features
  • Column eliminates approximately 94% of total protein (albumin, IgG, antitrypsin, IgA, transferrin, haptoglobin, fibrinogen, alpha2-macroglobulin, alpha1-acid glycoprotein, IgM, apolipoprotein Al, apolipoprotein All, complement C3, and transthyretin) when used with the Multiple Affinity Removal System.
  • Three column dimensions are available and include 4.6 x 50 mm, 4.6 x 100 mm, and 10 x 100 mm. Custom sizes and spin cartridges are also available.
  • Serum injection volume ranges between 20 - 40 µL per run for the 4.6 mm ID, and 160 µL for the 10 mm ID column.
  • Optimize sample recovery by utilizing our rapid, single injection protocols and optimized buffers.
  • Optimized 2 buffer system allows for highly reproducible re-use of the Columns for over 200 injections – available in a convenient Column Reagent Starter Kit
  • Process up to 8,000 µL of plasma/serum over the column lifetime for the 4.6 mm ID columns and 32,000 µL for the 10 mm ID columns.
  • Protocols and columns are optimized for use with human physiological samples. Column not recommended for use with mouse serum.
  • Column is compatible with most FPLC or HPLC pumps or instruments, including the Agilent 1200/1100 instruments.

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