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Performance of the Agilent Small RNA

Abstract: Small nucleic acids ranging in size from 6 to 150 nucleotides can be analyzed running the Small RNA assay on the Agilent 2100 bioanalyzer. This Technical Note describes the performance of the Small RNA assay. The Small RNA assay can: • Visualize miRNA, Small RNA, oligo nucleotides from 6 - 150 nt for verifying sample integrity • Quantify miRNA in the concentration range of 50 - 2000 pg/μL relative to an external standard, for verifying sample enrichment and purity • Automate sample quantitation, sizing and purity determination

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