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Genome-wide Monitoring of CpG Island Methylation With Agilent's Tiling Microarray Technology
Abstract:
DNA methylation profiling has emerged as an important technique for understanding the role methylation plays in cellular processes and as
a potential diagnostic and prognostic indicator in diseases such as cancer.
Of increasing importance is the ability of the researcher to observe genome-wide methylation patterns in multiple samples. We have combined tiling CpG Island
Microarrays with the methyl DNA immunoprecipitation method (mDIP;Weber et al., 2005) to compare relative methylation patterns across CpG islands
in two model systems — X-chromosome inactivation and cancer profiling. This application note introduces the protocol and highlights data generated.
Keywords:
DNA Methylation, CpG Island, CpG, CG-rich, CG, methylation, mDIP, 2-set, 5989-6560EN, G4492A, G4489A, G4490A, epigenetic, methylated
Publication Number:
5989-6838EN
Last Updated:
6/13/2007
Number of Pages:
8
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(0.27 MB)