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Improving the Effectiveness of Method Translation for Fast and High Resolution Separations
Abstract:
The increased availability of sub-2-micron (STM) columns and increased demand for methods friendly to mass spectrometers has led to strong trend toward conversion of existing HPLC methods to smaller diameter and smaller particle size columns. While the conversion is a simple mathematical exercise requiring the scaling flow rates, gradient times and injection volumes, many users observe less than perfect results. Here we look closely at the problem and propose calculations that improve the speed and/or resolution in a more predictable and beneficial way
Keywords:
herbicides, environmental, food, food safety, forensic, EPA Method 555, Zorbax, RRHT, 1200 SL, RRLC, Rapid Resolution LC, bandspreading, method translation, STM, sub-2-micron, van Deemter, Technology
Publication Number:
5989-5177EN
Last Updated:
11/2/2009
Number of Pages:
6
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(0.16 MB)