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Achieving fastest analyses with the Agilent 1200 Series Rapid Resolution LC system and 2.1-mm id columns

Abstract: The need to increase the daily throughputs of LC systems is a constant desire. Now, with the Agilent 1200 Series Rapid Resolution LC system highest throughputs are possible, and in combination with the Agilent ZORBAX RRHT columns and the increased pressure and temperature range of the LC system, excellent chromatographic resolution can be achieved even at run times below one minute. This Application Note describes the correct set-up of the instrument which is the key for optimal results with narrow bore columns, such as a 2.1 mm x 50 mm column packed with sub two micron particles. Peak capacities in the range of fifty in analysis times as short as 24 seconds and peak widths as narrow as 200 milliseconds are shown. The well-balanced use of all possible module options to achieve shortest cycle times with throughputs far beyond 1500 samples per day is described.

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