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Transfer of HPLC methods to Capillary Electrochromatography

Abstract: Capillary Electrochromatography combines the separation principle of HPLC (partitioning between mobile and stationary phases) with the high efficiency of capillary electroseparation methods in CEC. The electroosmotic flow (EOF) inherent in capillary electrophoretic separations is used to transport solute and mobile phase through a packed capillary column. The properties of the EOF allow much higher efficiencies than can be realized with LC. CEC can also be characterized by its low solvent consumption and reduced costs. Therefore, there are a number of advantages which may be gained by transferring a method from LC to CEC. This application brief aims to identify the parameters which must be appreciated in order to successfully transfer a method from LC to CEC.

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