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Liquid Chromatography

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Techniques of HPLC 


2300 (USD) H1186A  Day(s) Hands-On Operation 
2700 (CAD)
Description:

Training Units   23 (US)  |  27 (CA)         What are Training Units?

This hands-on laboratory and lecture course is designed to increase the participants understanding and ability to apply the modern practices of High Performance Liquid Chromatography. The participant will learn how to select appropriate instrumentation and HPLC columns, optimize separations, and interpret and troubleshoot chromatograms. Participants will learn how theoretical considerations will help increase their ability to produce robust methods and solve analytical problems. HPLC practices and evaluation of system performance will help students increase their efficiency and data quality. The hands-on aspect of the course will allow a novice HPLC user to gain important practical operational experience with the HPLC.

Outline:
Day One
Introduction to HPLC
HPLC Instrumentation
Day Two
Practical HPLC Requirements
The Separation
Gradient Elution
Day Three
HPLC Troubleshooting
Quantitative and Qualitative Analysis
Column Hardware
Reversed-Phase HPLC
Day Four
Ion-Pair HPLC
Ion Exchange
Normal Phase
Size Exclusion
Method Development
Method Validation
Prerequisites:
None; however, a working knowledge of PC's is recommended.
Equipment: The Agilent 1100/1200 Series Liquid Chomatographs with HPLC ChemStation  
Student Profile: A neophyte user of Agilent liquid chromatographs who has responsibility for routine sample analysis and may have responsibility for developing new methods.  

Course Calendar:
IL, ChicagoApril 17, 2012 - April 20, 2012
GA, AlpharettaMay 21, 2012 - May 24, 2012
IL, ChicagoAugust 13, 2012 - August 16, 2012
GA, AlpharettaNovember 27, 2012 - November 30, 2012
QC, Ville St. LaurentApril 24, 2012 - April 27, 2012

To register your interest in a training course simply click on the relevant city / date or  the "On Demand" link below, if no dates are listed.

On Demand

Follow-on Courses:

  • H4039A - Agilent HPLC (3D) ChemStation Data Analysis and Reporting
  • H5928A - Agilent HPLC (2D) ChemStation Data Analysis and Reporting
  • H4033A - Agilent HPLC (3D) ChemStation Operation
  • H8969A - Agilent 1100/1200 HPLC Troubleshooting and Maintenance

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