The Agilent 7000 Series Triple Quadrupole GC/MS system was utilized to develop a rapid method for screening more than 150 doping agents in seven classes of substances, at or below World Anti-Doping Agency Minimum Required Performance Levels (WADA MRPLS). A short capillary column, rapid scan speed and hydrogen as carrier gas enables a run time of less than eight minutes. The method was developed on an Agilent 7890 gas chromatograph equipped with a split/splitless capillary inlet and an Agilent 7000A Triple Quadrupole GC/MS system, using a Gerstel MPS2 autosampler and PTV injector. The method is comprehensive, encompassing one or more metabolites of all prohibited narcotics, the most frequently used ß2-agonists, hormone antagonists and modulators, and beta-blockers.
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