1200 Series - Sensitivity and Performance 

Optimized to give you complete confidence in your results

Spectral information from 190 to 950 nm
The dual-lamp design and the temperature control of the Agilent 1200 Series diode-array detectors ensure outstanding sensitivity. Photodiode arrays with 1024 elements, programmable slit width (1, 2, 4, 8, 16 nm) and the selection of nine different flow cells enable optimization of resolution, linearity and sensitivity. See Agilent 1200 Series diode-array detector and Agilent 1200 Series diode-array detector SL.

Mass selective detection for more confidence
By providing molecular weight and structural information, which complements the spectral information from a diode array detector, the Agilent 6000 Series LC/MS systems are the ideal solutions for scientists who need additional selectivity and sensitivity for their chromatographic systems. See LC/MS systems.

HPLC-Chip/MS for reliable high sensitivity nanospray
The easy-to-use HPLC-Chip/MS system significantly improves your nanoflow separations by eliminating all post-column dead volumes and is ideally suited for high sensitivity nanospray LC/MS with limited sample amounts. See Agilent 1200 Series HPLC-Chip/MS system.

Superior injection precision and lowest carryover
The selection of the 1200 Series autosamplers offer precision levels of < 0.5 % RSD over an injection volume range from 0.01 µL to 2000 µL. The unique flow-through design, combined with the external needle wash and optional multiple wash solvents, virtually eliminates carryover, even for highly adsorptive compounds. See also Agilent 1200 Series autosamplers.

High resolution chromatography reveals more
Small particle size and long separation columns are key for higher efficiency and resolution. The Agilent 1200 Series Rapid Resolution system fully exploits the power of Agilent ZORBAX Rapid Resolution HT columns with 1.8 µm particles, providing a new dimension of information for complex samples.

Agilent 1200 Series LC Typical Performance

• Injection volumes from 30 nL up to 5 mL
• Sample carryover as low as 0.003%
• Retention time precision as low as 0.1 % RSD
• Total cycle time as short as 66 seconds for gradient runs
• UV - limit of detection as low as 10 pg anthracene
• FLD- limit of detection as low as 50 femtomole amino acid
• AP-MALDI/Ion Trap: Limit of detection as low as 125 amol of BSA tryptic digest (MS only)

Agilent 1200 Series Rapid Resolution LC/Ultra-Fast LC Performance

• More than 20 x faster compared to HPLC
• 60% resolution gain compared to HPLC
• Less than 0.1% RSD typical retention time precision
• Less than 0.5% RSD typical area precision
• 0.03% level impurities quantification
• Less than 1 pg LOD of anthracene
• Compatibility with conventional HPLC methods
• Method, instrument and column robustness in ultra-fast LC
• Peak purity analysis of trace level side compounds under ultra-fast LC conditions
• Spectral confirmation of trace level side compounds under ultra-fast LC conditions
• Resolution gain in ultra-fast LC by 80 Hz data acquisition

Agilent 1200 Series Capillary LC Performance

• Influence of decreased column id on the limit of detection
• Precision of retention times
• Impact of backpressure fluctuations on retention times
• Gradient performance - composition accuracy, precision and mixing noise
• The Agilent 1200 Series micro well-plate sampler - Precision of areas
• The Agilent 1200 Series micro well-plate sampler - Injection volume linearity
• Limit of detection using DAD, MSD and MSD Trap
• The Agilent 1200 Series capillary LC system - modified flow rates above 0.1 mL/min

Agilent 1200 HPLC-Chip/MS Performance

• Sensitivity
• Chromatographic performance
• Reproducibility
• Nanospray stability

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