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USP Designations 

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The US Pharmacopeia (USP) is a standard source for many pharmaceutical methods. The USP specifies columns by packing materials rather than by manufacturer. Listed below are the recommended Agilent Technologies HPLC columns suitable for most LC methods listed with the USP.

Click on the column phase for additional information and ordering.

USP Packing Materials

Columns

Particle Size (µm)

Pore Size (Å)

L1

Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 3 to 10 µm in diameter

ZORBAX Eclipse Plus C18
ZORBAX Eclipse XDB-C18
ZORBAX SB-C18
ZORBAX Rx-C18
ZORBAX Extend-C18
ZORBAX ODS
ZORBAX ODS Classic
LiChrosorb RP-18
LiChrospher RP-18
Purospher RP-18/-e
Superspher RP-18/-e
Nucleosil 100-5 C18

3.5, 5
3.5, 5
3.5, 5
3.5, 5
3, 5
5
5
5, 10
5
5
4
5

95
80, 300
80, 300
80, 300
120
70
70
100
100
100
100
100

L2

Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50 µm in diameter

N/A

L3

Porous silica particles, 5 to 10 µm in diameter

ZORBAX SIL
ZORBAX Rx-SIL
LiChrospher 60 Si

5
3.5, 5
5

70
80, 300
60

L4

Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter

Custom Order

L5

Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter

N/A

L6

Strong cation-exchange packing: sulfonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 µm in diameter

N/A

L7

Octyl silane chemcially bonded to totally porous microsilica particles, 5 to 10 µm in diameter.

ZORBAX C8
ZORBAX Eclipse Plus C8
ZORBAX Eclipse XDB-C8
ZORBAX SB-C8
ZORBAX Rx-C8
LiChrosorb RP-8
LiChrospher RP-8
LiChrospher RP select B

5
3.5, 5
3.5, 5
3.5, 5
3.5, 5
5
5
5

70
80
80, 300
80, 300
80, 300
100
100
60

L8

An essentailly monomolecular layer of aminopropyl-silane chemcially boneded to totally porous silica gel support, 10 µm in diameter.

ZORBAX NH2
LiChrospher NH2

5
5

70
100

L9

10 µm irregular, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating.

ZORBAX SCX
SynChropak SCX

5 spherical
6.5

300
300

L10

Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter.

ZORBAX CN
ZORBAX SB-CN
ZORBAX Eclipse XDB-CN
LiChrospher CN

5
3.5, 5
3.5, 5
5

70
80, 300
80
100

L11

Phenyl groups chemically bonded to porous silica particles, 5 to 10 µm in diameter.

ZORBAX Phenyl
ZORBAX SB-Phenyl
ZORBAX Eclipse XDB Phenyl

5
3.5, 5
3.5, 5

70
80
80

L12

Strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 5 µm in diameter

Accubond Bulk SAX, 25 g bottle

L13

Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter.

ZORBAX TMS

5

70

L14

Silica gel 10 µm in diameter having a chemically bonded, strongly basic quaternary ammonium anion-exchange coating.

ZORBAX SAX
SynChropak SAX

5
6.5

70
300

L15

Hexyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter

N/A

L16

Dimethyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter.

N/A

L17

Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 µm in diameter.

N/A

L18

Amino and cyano groups chemically bonded to porous silica partices 5 to 10 µm in diameter.

N/A

L19

Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 9 µm in diameter.

N/A

L20

Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10 µm in diameter

LiChrospher Diol

5

100

L21

A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm in diameter.

N/A

L22

A cation group resin made of porous polystyrene gel with sulfonic acid groups, about 10 µm in size.

N/A

L23

An ion exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 10 µm in size.

N/A

L24

A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface, 32 to 63 µm in diameter.

N/A

L25

Packing having the capacity to separate compounds with a MW range from 100 to 5000 da (as determined by polyethylene oxide), applied to neutral, anionic and cationic water-soluable polymers.

PL aquagel-OH

L26

Butyl silane chemically bonded to totally porous silica particles, 5 to 10 µm in diameter.

N/A

L27

Porous silica particles, 30 to 50 µm diameter

AccuBond Bulk SIL, 25 g bottle

L28

A multifunctional support, which consists of a high purity, 100Å, spherical silica substrate that has been bonded with anionic (amine) functionality in addition to conventional reversed-phase C8 functionality.

N/A

L29

Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5µm diameter with a pore diameter of 80Å.

N/A

L30

Ethyl silane chemically bonded to a totally porous silica particle, 3 to 10 µm in diameter

N/A

L31

A strong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5 µm macroporous particles having a pore size of 2000Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene.

N/A

L32

A chiral ligand-exchange packing- L-proline copper complex covanlently bonded to irregularly shaped silica particles, 5 to 10 µm in diameter.

N/A

L33

Packing having the capacity to separate proteins by molecular size over a range of 4,000 to 400,000 daltons. It is spherical, silica-based, and processed to provide pH stability.

ZORBAX GF-250

4

150

L34

Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, 9µm in diameter.

N/A

L35

A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase

ZORBAX GF-250
ZORBAX GF-450

4
6

150
300

L36

L-Phenylglycine-3, 5-dinitrobenzoyl on 5 µm amino propyl silica.

N/A

L37

Polymethacrylate gel packing having the capacity to separate proteins by molecular size over a range of 2,000–40,000Da MW

N/A

L38

Methacrylate-based size exclusion packing for water-solubles

N/A

L39

Hydrophilic polyhydroxymeth-acrylate gel of totally porous spherical resin

N/A

L40

Cellulose tris-3,5-dimethylphenylcarb-amate coated porous silica particles, 5 to 20 µm in diameter

N/A

L41

Immobilized alpha-acid glyco-protein on spherical silica particles, 5 µm in diameter

N/A

L42

Octlysiilane and octadecylsilane groups chemically bonded to porous silica particles

N/A

L43

Pentafluoropehnyl groups chemically bonded to silica particles 5 to 10 µm in diameter

N/A

L44

A multifunctional support, which consists of a high purity, 60Å spherical silica substrate, that has been bonded with a cationic exchanger, sulfonic acid functionality in addition to a conventional reversed phase C8 functionality.

N/A

L45

Beta cyclodextrin bonded to porous silica particles, 5 to 10 µm in diameter.

ChiraDex Chiral

5

L46

Polystyrene/divinylbenzene substrate agglomerated with quarternary amine functionalized latex beads, 10µm in diameter.

N/A

L47

High capacity anion exchange microporous substrate, fully functionalized with a trimethyl-amine group, 8 µm in diameter.

N/A

L48

Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous, anion-exchange microbeads, 15 µm in diameter.

N/A

L49

Amylose tris-3, 5-dimethylphenyl-carabamate-coated, porous, spherical, silica particles, 5 to 10 µm in diameter.

N/A

L50

A strong cation exchange resin made of porous silica with sulfopropyl groups, 5 to 10 µm in diameter.

ZORBAX 300SCX
SynChropak SCX

5
5

300
300

L51

A reversed-phase packing made by coating a thin layer of polybutadiene on to spherical porous zirconia particles, 3 to 10 µm in diameter.

N/A

L52

Multifunction resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinyl-benzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 µm in diameter, and a surface of not less than 350m2/g, substrate is coated with quarternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene.

N/A

L53

An anion-exchange resin consisting of rigid, spherical styrene-divinylbenzene copolymer with trimethylammonium groups at a loading of about 2meq per g, 3 to 29 µm in diameter.

N/A

L54

Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 7 to 11 µm in diameter.

N/A

L55

Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinyl-benzene copolyme, 3 to 15 µm in diameter. Substrate is surface grafted with carboxylic acide and/or phosphoric acid functionalized monomers. Capacity not less than 500 µeq/column.

N/A

L56

Propyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter

SB-C3

3.5, 5

80

L57

A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with a pore size of 120Å

Ultron ES-OVM

5

120

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