LC & LC/MS Columns 

Analytical HPLC & UHPLC 

ZORBAX Eclipse Plus 
Product Description
Eclipse Plus columns provide the ultimate in performance for silica-based columns. Peak shape for the Eclipse Plus is excellent for the most challenging basic compounds, improving efficiency and resolution with these sample types. These results are achieved by improvements in the silica manufacturing and bonding technology, which is completely controlled by Agilent. Eclipse Plus columns are the ideal first choice for method development of all samples. To achieve fast method development and superior productivity, choose an Eclipse Plus column with high-resolution 1.8 µm particles. For standard methods, conventional 5 µm Eclipse Plus Columns and Rapid Resolution 3.5 µm Eclipse Plus columns are your best choices. With all particle sizes, easy method transfer is possible.
The EC-C18, EC-C8 and Phenyl-Hexyl phases on Poroshell 120 are very similar to Eclipse Plus C18, Eclipse Plus C8 and Eclipse Plus Phenyl-Hexyl phases. See more on Poroshell 120.

  • The ideal column for method development – excellent results for a wide range of compounds
  • High level of performance – peak shape, efficiency, resolution, and lifetime – with all sample types: acids, bases and neutrals
  • Superior reproducibility with more rigorous QA/QC testing
  • Improved, patented silica manufacturing with start-to-finish product control
  • Available in 1.8, 3.5, and 5 μm particle sizes for all analytical, high resolution, and fast LC analyses
  • To compare technical information and specifications for different phases, see our phase specifications chart.
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  • Eclipse Plus columns are recommended for initial method development from pH 2-9 and provide high resolution and excellent peak shape of all types of compounds. Like all Eclipse columns Eclipse Plus columns achieve superior performance through extra dense bonding and a precise double-endcapping process. But Eclipse Plus columns also feature enhanced high-performance silica, new endcapping reagents, and a unique bonding process that is optimized for the improved silica.

    Eclipse Plus columns are available in C18, C8, Phenyl-Hexyl and a C18 bonded phase for the separation of PAHs (polycyclic aromatic hydrocarbons). These phase choices provide the flexibility to rapidly separate all types of compounds including achieving superior resolution with aromatic compounds using the Eclipse Plus Phenyl-Hexyl. Eclipse Plus columns are available in particle sizes ranging from 1.8 µm to 5 µm RRHT to Prep and are a rapidly growing new product family. Rapid Resolution HT (RRHT) 1.8 µm columns provide the fastest analysis times and the highest resolution in a new wider range of column dimensions supporting high pressure use.

    ZORBAX Eclipse Plus Column Specifications

    Bonded Phase Pore Size Surface Area Temp. Limits pH Range* End capped Carbon Load USP Designation
    ZORBAX Eclipse Plus C18 95Å 160 m2/g 60 °C 2.0 - 9.0 Double 9% L1
    ZORBAX Eclipse Plus C8 95Å 160 m2/g 60 °C 2.0 - 9.0 Double 7% L7
    ZORBAX Eclipse PAH 95Å 160 m2/g 60 °C 2.0 - 8.0 No 14% L1
    ZORBAX Eclipse Plus Phenyl-Hexyl 95Å 160 m2/g 60 °C 2.0 - 8.0 Double 9% L11

    Specifications represent typical values only.

    *Column lifetime will be reduced significantly at pH >7 and temperature > 40 °C. At pH 6-9, highest column stability for all silica based columns is obtained by operating at temperatures < 40 °C and using lower buffer concentrations in range of 0.01 0.02M, especially with phosphate and carbonate buffers.

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