KB001998: What LC Cleaning Solvent Mixture can be Used to Steam Clean a LC/MS System? - General LC/MS 

One of the solvent mixture found to be very valuable in cleaning LC systems, as well as LC/MS interfaces like ESI, consists of the following components: 25%:25%:50% Cyclohexane/Acetontirile/Isopropanol. However, this solvent mixture is used only for rinsing / steam cleaning and should not remain in the LC system for an extended period of time. It has been reported that cyclohexane may be harmful to the LC pump seals. Therefore, make sure to remove the solvent mixture from the system after the cleaning procedure is finished by flushing with IPA. Note: It is advised to use a union in place of column,.