KB004939: Why and How is the "Corrected RNA Area" Determined? - 2100 Expert Software 

This correction stems from the fact that since all bands pass the detector at different speeds, the area (which is used for quantification) is time dependent. To correct for this effect, we divide the measured signal by the migration time for each data point. That way we get normalized areas that can be used for the calculation of RNA concentration. This is done in all other electrophoretic assays.