The protein concentration in the Protein 80 and Protein 230 assay is determined relative to the upper marker of known concentration, which is included in each sample.
For the High Sensitivity Protein 250 assay, the relative quantification is based on the seven ladder peaks. The concentration of the ladder is 5000 pg/µL. The software automatically calculates the concentration of the sample. Multiply the result with the dilution factor (e.g. x 200) to receive the concentration of the initial sample solution. It is possible to adjust the ladder concentration in the software, in case a different dilution for sample and ladder are used. This adjustment can also be used to correct for different labeling efficiency of ladder and sample.
In addition to the relative quantification it is also possible to perform an absolute protein quantification based on user defined standards and a calibration curve for all protein assays.