KB005020: There are no cell events detected? 

Please check the cell concentration in your sample with a counting chamber to make sure the cell concentrations is high enough. Visually inspect sample well under microscope to confirm that cells where loaded correctly. In addition, check staining procedure. Always prepare positive and negative staining control. The staining protocol (dye concentration, incubation time, temperature,...) may need optimization for the specific cell preparation. For application specific protocols and recommended staining reagents please refer to the application notes available from our webpage.