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Please select a topic from this section below:
Capillary Electrophoresis
LC and LC/MS Columns
LC Chromatography Troubleshooting
LC Column Troubleshooting
LC Method Development and Transfer
Click a question below to see the answer:
Brief explanation for Retention Time, Retention Factor (k), Retention Index (I), and Separation Factor(α)
Brief explanation for Number of Theoretical Plates (N), Height Equivalent to a Theoretical Plate (H), Utilization of Theoretical Efficiency (UTE%), Resolution (RS), and Phase Ratio (β)
I received a new HPLC column from Agilent, but it has no end fittings. How do I connect it to my instrument?
What should I do before using my brand new RPLC column for the first time?
What are the advantages of using a 10 micron particle size preparative column?
If particle size is not critical for a preparative column, is the choice of bonded phase?
I want to perform a loading study for my analytes that I want to isolate and purify, what size column should I use?
I generally need to purify no more than 50 mg of sample at one time. Can I select one column size and eliminate the loading studies?
Scaling HPLC separations is most directly related to which column parameter?
What about scaling gradient separations ? Do you scale using column volume?
How to replacement of the capillary cutter (P/N:5183-4669) blade (P/N:5183-4670) ?
What is HILIC?
How Does HILIC Work?
What type of the LC columns are suitable for the phases mentioned in USP methods?
What are STM Columns and their advantages?
Can I transfer easily the analysis method conditions done on short Rapid Resolution High Throughput (RRHT) columns to longer 3.5 and 5 um columns used in analytical mode?
Can you provide an example of application of SB-phenyl Rapid Resolution High-throughput columns and how these columns are of advantage for us?
Can you provide an example how to make use of RRHT Columns with Different C18 Selectivity’s to Quickly Develop Methods?
Can you provide me an example on how I can use my Agilent 1100 series HPLC system for Ultra Fast Liquid Chromatography applications?
Can I use my Agilent 1100 series HPLC system for alternating column generation applications?
Can you provide me an example on how I can use my Agilent 1100 series HPLC system for alternating column generation applications?
Can you provide details on some of the tubing volumes used in HPLC?
How to Update Existing Methods for Fast or Ultra-Fast Analysis with Rapid Resolution HT Columns?
Can you provide me comparision between some of the general purpose and Zorbax RRHT columns?
Can you provide an example of high speed Amino Acid Analysis on 1.8um Reverse Phase columns?
