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KB000022: Which Guard Column / Hardware Should be Used for ZORBAX Standard and Agilent Cartridge Columns? - LC Column 

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  • Which Guard Column / Hardware Should be Used for ZORBAX Standard and Agilent Cartridge Columns?
      Answer: ZORBAX Columns with ZORBAX Hardware Except in special circumstances, you should try to match exactly the guard column with the same packing and internal diameter as the column you're trying to protect. Find the part number and description of the column you have (or are ordering) on the Agilent web site or in our catalog or column selection guide. Find the part number and description for a guard column with the same (or less retentive) packing material. Select the part number for the appropriate guard column, based on whether the column is an analytical (4.6 mm, 4.0 mm, 3.0 mm Solvent Saver), narrow-bore (2.1 mm), semi-preparative (9.4 mm) or preparative (21.2 mm) column. For example, if your column is an analytical column, 4.6 mm x 150 mm, 3.5-micron Eclipse XDB-C8, you should select an XDB-C8 analytical guard cartridge, with dimensions 4.6 mm x 12.5 mm. If you're using a 2.1-mm i.d. narrow-bore column, select a narrow-bore 2.1 x 12.5 mm guard column. Both of these guard columns use the same hardware kit (820888-901). ZORBAX PrepHT columns (21.2 mm i.d.) have their own integral guard columns and have hardware for the inlet end for use of a guard cartridge and the end fittings. See Table 1 for several specific examples.
      Table 1 Guard Column and Hardware Selection
      Column Description Column Part Number Guard Column Description Guard Part Number Guard Hardware Part Number
      Eclipse XDB-C8
      4.6 x 150 mm, 3.5-um
      analytical column
      963967-906 Analytical
      4.6 x 12.5 mm
      5-um
      820950-926 820888-901
      Eclipse XDB-C8 2.1 x 150 mm, 5-um narrow-bore column 963967-912 Narrow-bore 2.1 x 12.5 mm 5-um 821125-926 820888-901
      StableBond SB-C18 21.2 x 250 mm, 7-um
      PrepHT column
      877250-102 PrepHT guard column 17 x 7.5 mm 5-um 820212-902 820444-901
      (guard hardware) 820400-901
      (end fittings)
      Columns Having the Agilent Designation For ZORBAX and other manufacturers' columns that are sold in an Agilent cartridge format, catalog entries are designated by 'AC' in white letters within a black diamond. For Agilent cartridge columns you must purchase the guard cartridges separately in the given packing material. But the guard cartridge holder is an integral part of the Agilent cartridge column hardware for 2, 3, 4 and 4.6 mm i.d. configurations. The cartridge hardware kit consists of two end fittings and two sets of stainless steel collets. When the cartridge column is used without a guard column, the inlet end collets are installed in one direction; when the cartridge column is used with a guard column, the collets are installed in the reverse direction to allow the 4 x 4 mm guard cartridge to fit within the inlet column cartridge holder. Replacement filters may be used to protect the cartridge column from particulates (part no. 5063-6574 for 4 and 4.6 mm cartridges, part no. 5063-6519 for 2 mm cartridges), but are not required. A mounting tool (part no. 5021-1846) is also needed for installing the replacement filters, and is included in the cartridge holder kit (part no. 5021-1845).
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