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KB005494: To analyse/compare profile data of different platforms for single experiment. GeneSpring GX. 

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  • I want to analyse and compare profile data from different platforms for a single experiment. I have an Affymetrix data (test) and Agilent data (control). Shall I be able to compare and analyse them?

      To compare the data from two technologies within a project, Translation feature could be used. This feature allows comparison of entity lists between experiments of different technologies.
      Translation is performed using Entrez Gene ID. Please find the steps below to perform Translation:

      1. Two experiments of different technologies should be open e.g., A1 (Source) & A 2(Target)
      2. Experiment A2 should be active
      3. Right click on the entity list to be translated in Experiment A1 -> Select 'Translate list' option
      4. Please follow the ‘Translation Inspector’ Wizard.

      The translated list from experiment A1 is now seen in the Project Navigator of active experiment A2.
      Please find the link below for animated tutorial on Translation in GeneSpring GX 10:
      http://www.chem.agilent.com/en-US/products/software/lifesciencesinformatics/pages/translationgx10_viewlet_swf.aspx  

      Also, the ‘Venn diagram’ functionality could be used to automatically translate and combine the entity lists from two different technologies.

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