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Fast Method Development of Salicylic Acid Process Impurities using Agilent ZORBAX Rapid Resolution High Throughput Columns with the Agilent 1200 Series Method Development Solution Controlled by AutoChrom Version 12.01

Abstract: A sample consisting of salicylic acid and its published process impurities are separated using a method developed on an Agilent 1200 Series Method Development Solution controlled by ACD/AutoChrom software, version 12.01. The chromatograph while under control of AutoChrom can screen up to seven columns, 13 buffers and two organic solvents with columns held in four temperature-controlled zones. The software helps plan the next best experiment to perform, allowing the analyst to focus on quickly developing methods using conditions with the best likelihood of success. In this work three columns (Agilent ZORBAX StableBond SB-C18, ZORBAX Eclipse Plus C18 and StableBond SB-Aq) are screened using five mobile phase modifiers. Temperature is maintained at 25 °C throughout the experiment. Fifteen solvent column experiments are initially screened. Several experiments follow to construct a retention model. A solution is achieved with a final in under 3 min isocratic separation.

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