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Fast Separation of Seven Biocides Using an Agilent ZORBAX Rapid Resolution High Definition Eclipse Plus C18 1.8 μm Column
Abstract:
An Agilent ZORBAX Rapid Resolution High Definition (RRHD) Eclipse Plus C18 1.8 μm column separated seven different biocides rapidly with high resolution. The data presented
illustrate the capability of the short 50 mm, 1.8 μm column to maintain high resolution of complex samples at very fast flow rates and high pressures, up to 1020 bar. The high quality separation was maintained when the method run was reduced
from 3 minutes to 0.7 minutes by increasing the flow rate from 1.0 mL/min to 1.7 mL/min. The Agilent 1290 Infinity LC System was used because the column pressure
just exceeded 1000 bar at this high flow rate. Methylparaben, listed as an echinacea supplement ingredient, was easily identified and a hand sanitizer containing
2-phenoxyethanol and methylparaben were also easily and rapidly identified using the method.
Keywords:
ZORBAX, methylparaben, echinacea, RRHD, 1290, Eclipse Plus,
Publication Number:
5990-4899EN
Last Updated:
11/2/2009
Number of Pages:
8
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