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Rapid Peptide Mapping Method with High Resolution Using a sub 2-μm Column
Abstract:
A high-speed method was developed for peptide mapping that offers a high degree of column resolution and excellent retention time and peak area reproducibility. The
method uses a sub 2-μm column that gives complete sequence coverage and a reduction in run time from 214 min obtained with conventional 5-μm particle columns, to 60
min with no loss in peak capacity. A flow rate of 0.5 mL/min allows the use of MS detection to yield quality mass spectral data for peptide identification and gives the
analyst the versatility of using conventional HPLC instrumentation without the need for high-pressure pumps.
Keywords:
peptide, SB-C3, peptide mapping , ZORBAX, protein digest, sub-2 micron,
Publication Number:
5990-4712EN
Last Updated:
10/15/2009
Number of Pages:
6
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(1.14 MB)