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Peptide Mapping of a Monoclonal Antibody using a Microfluidic-based HPLC-Chip coupled to an Agilent Accurate-Mass Q-TOF LC/MS

Abstract: The sequence characterization of a monoclonal antibody (mAb) at low nanogram levels using an Agilent HPLC-Chip system coupled to an Agilent Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS instrument is described. The exact mass measurement provided by this high performance Q-TOF LC/MS ensures confidence when determining analytes such as peptides. The superior sensitivity and mass accuracy of the HPLC-Chip LC/MS platform, combined with the powerful data processing capabilities of Agilent MassHunter and BioConfirm software, enabled complete sequence coverage of heavy and light chains and enabled mass confirmation of all identified peptides within a 4 ppm mass accuracy. The robust, reliable, and easy to use Agilent HPLC-Chip/MS System, which has significant benefits for peptide analyses, is ideally suited for the routine analysis of small quantity biopharmaceuticals.

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