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Automated Sample Preparation for Profiling Fatty Acids in Blood and Plasma using the Agilent 7693 ALS
Abstract:
A miniaturized automated sample preparation method is described for the determination of fatty acid methyl esters in blood or plasma samples using the Agilent 7693
Series Automatic Liquid Sampler. Fifty-microliter samples are derivatized using acetyl chloride in methanol and the fatty acid methyl esters are extracted in iso-octane. Extracts are analyzed by GC-FID. Excellent repeatability is obtained and the method can be applied for fully automated fatty acid profiling in blood and plasma samples during dietary, biomedical or metabolomic studies.
Keywords:
Life Science, lipidomics, fatty acid methyl esters (FAMEs), blood, GC-FID, automated sample preparation, 7693 ALS
Publication Number:
5990-4822EN
Last Updated:
10/9/2009
Number of Pages:
8
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