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Rapid Analysis of Vitamin D in Serum Using Triple Quadrupole LC/MS

Abstract: A rapid method for the quantification of 25-OH D2 and 25-OH D3 in serum was developed using the Agilent 1200 Series Rapid Resolution LC (RRLC) system coupled with an Agilent 6430 Triple Quadrupole LC/MS with multimode ionization (MMI). Solid phase extraction minimizes matrix interferences and ion suppression, while enabling automation. Excellent linearity of quantification (R2 > 0.99) was obtained in the range of 5 to 100 ng/mL, with run times of only 5 min, and day-to-day precision was well below 10%. The limits of detection (LOD) and limits of quantification (LOQ) were 2 ng/mL and 3 ng/mL respectively, in serum.

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