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Improved Sample Quality Control for the Next Generation Sequencing Workflow

Abstract: Agilent Technologies is the leader in commercial microfluidic Lab-on-a-Chip technology. The Agilent 2100 Bioanalyzer and RNA LabChip kits play an important role in assisting researchers in the determination of RNA quality. The RNA Integrity Number (RIN) has become the standard in RNA quality assessment. With the advent of next-generation sequencing (NGS) platforms, sizing, quantification and quality assessment of fragmented DNA starting material as well as DNA sequencing libraries has become essential to achieve highest quality sequencing data. Recommended by NGS platform providers, the Agilent 2100 Bioanalyzer has become the industry standard for automated sizing and quantification of various sample types relevant for the next generation sequencing sample preparation workflow. The Agilent High Sensitivity DNA Kit allows sizing and quantification of DNA samples in the single-digit pg/µL concentration range

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