Online literature is provided as Adobe Acrobat Portable Document Format (PDF) files. Obtain your free copy of the current Acrobat Reader from Adobe Systems.

Complete Automation of Quantitative Polymerase Chain Reaction Assays on the Agilent Bravo

Abstract: The Agilent Bravo Automated Liquid Handling Platform has been shown to be an effective way to automate setup of a variety of quantitative polymerase chain reaction (QPCR) assays. QPCR reactions to examine a variety of housekeeping genes from cDNAs derived from different human tissues were set up on the Bravo Automated Liquid Handling Platform and by hand. Dilutions performed by each method showed similar slopes and good linearity and targets amplified by either method also gave similar Ct values. When examining cDNAs from fetal and adult lung, differential expression was observed in the B2M whereas GUSB and TBP remained similar. The Bravo Automated Liquid Handling Platform should be useful to scientists interested in speeding up setup while maintaining reliability when performing QPCR studies.

View Article (0.33 MB)

For your convenience this PDF file will open in a separate window, and you may need to adjust your pop up blocker setting.