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High-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System
Abstract:
The Agilent 1290 Infinity LC System with ultraviolet/visible (UV/VIS) Diode Array
detection (DAD) is used to analyze triglycerides in soybean oil under non-aqueous
reversed phase gradient conditions. The Agilent 1290 Infinity LC System was used for
the chromatographic separation of the sample on 3.0 and 2.1 mm id C18 columns, of
various lengths, with 1.8-μm packing materials prepared in 600 bar (9000 psi) or special
1200 bar (18,000 psi) configurations. The ability of the Agilent 1290 Infinity LC
System to operate with long, high resolution columns is demonstrated with isopropanol
(IPA) or methyl tert butyl ether (MTBE) as the strong solvent and acetonitrile
as the weak component of the mobile phase mixture.
Keywords:
HPLC, RRLC, UHPLC, triglyceride, lipid, metabolism, biofuels, biodiesel, Infinity, 1290, viscosity, RRHT, RRHD, Ethanol
Publication Number:
5990-4292EN
Last Updated:
11/5/2009
Number of Pages:
8
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