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Agilent One Color RNA Spike-in Kit Protocol

Abstract: The Agilent One Color RNA Spike-In Kit was developed to provide positive controls for monitoring the Agilent One Color Gene Expression microarray workflow from sample amplification and labeling to microarray processing. The Agilent One Color RNA Spike-In Kit consists of a set of 10 positive con-trol transcripts optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization. Many Agilent catalog and custom microarrays contain the comple-mentary Spike-In probe sets. The Spike-In probe sets were designed to not have complementary sequences to the biological samples used on most microarrays.

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