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This application note describes the development and validation of a method to quantify clozapine, and two of its metabolites, in rat whole blood using the Agilent AACE LC/MS system for the online analysis of DBS cards. The quantitative performance of the online extraction method is evaluated and ...
The Agilent Automated Card Extraction (AACE) LC/MS System is a fully integrated instrument for the automated analysis of Dried Blood Spots (DBS) and other dried media. The system consists of the AACE instrument which enables automated flow-through analysis of DBS cards, together with software to ...
Agilent’s SureSelectXT Human Methyl-Seq target enrichment platform provides a comprehensive, efficient, robust, and cost-effective method for sequencing subsets of the human methylome. Sequencing bisulfite converted DNA, following SureSelectXT Human Methyl-Seq target enrichment, allows for the ch...
Improve liquid/liquid extraction for bioanalysis studies with Chem Elut solid supported liquid extraction (SLE) products: Improve high-throughput metabolism, pharmacokinetic, and toxicological studies, get reproducible organic phase separation everytime, and use simple, general methods for all sa...
Purification of synthesis mixtures is easy and fast with Varian’s Hydromatrix brand diatomaceous earth. This highpurity support improves purification compared to traditional liquid/liquid extraction by eliminating emulsion formation and improving purification reproducibility. Hydromatrix is packe...
Environmental analysis is plagued by difficult sample preparation. Homogenates of samples containing lipids or other fats readily form emulsions. Other solid samples such as soil and sludge are not readily homogenized and can be difficult to extract directly. Fortunately, these problems are avoid...
An Agilent 7890 Series GC was configured to run the newly revised European method EN14103:2011 for the determination of FAME content in finished B100 biodiesel. Four different types of biodiesel samples were manually prepared and analyzed using this system. For each biodiesel type, the total FAME...
Chinese Hamster Ovary (CHO) cells are commonly used as a host system for the production of human therapeutic proteins. An important part of the purification process is the removal of host cell material such as DNA, which poses a safety concern if administered along with the therapeutic compound. Q...
Dried matrix spotting (DMS) or dried blood spotting (DBS) technology, combined with the analytical capability of modern mass spectrometers (LC/MS/MS), has recently emerged as an important method in the quantitative bioanalysis of small molecules. The great interest in DBS lies in the small volume...
The hydrolysis of 2,4-dinitrophenol acetate by sodium hydroxide has become a standard reaction for testing the performance of fast kinetic instruments. The reaction was fi rst published by Gutfreund in 1969 and can be measured with an Agilent Cary 50/60 UV-Vis spectrophotometer and SFA 20 stopped...
A new and rapid qualitative method for detection of drugs in whole blood was developed using the innovative thermal separation probe (TSP) sample introduction technique on the transportable Agilent 5975T LTM GC/MS. No sample cleanup was required, and Deconvolution Reporting Software (DRS) softwar...
Normal phase liquid chromatography (LC) methods often have long run times and involve environmentally toxic/costly solvents. Supercritical fluid chromatography (SFC) methods on the other hand are faster, inexpensive, and eco-friendly. SFC involves the use of low viscosity supercritical carbon dio...
With multiple lots testing on headspace GC/MS, the new High Performance headspace (HS) septa were demonstrated to provide contamination free sample analysis at various HS temperature (up to 300 °C) and satisfactory chemical compatibility with common HS solvents. The High Performance HS septa are ...
Aluminium oxide (Al2O3), commonly known as alumina, is a widespread naturally occurring compound that has a broad range of industrial applications. Besides its use as a pigment or abrasive, alumina also plays a vital role as catalyst support for desulfurization or hydrogenation reactions. In the ...
Rapid and accurate methods are needed to quickly detect mycotoxigenic fungi such as those in the genera of Aspergillus, Penicillium, and Fusarium. This study was conducted to develop and test a multiplex real-time qPCR assay to detect and quantify these agriculturally and economically important f...
The Agilent 385-ELSD is an advanced evaporative light scattering detector that delivers subambient evaporation down to 10 °C, providing maximum sensitivity for compounds with significant volatility below ambient temperature. The 385-ELSD benefits from fast data output rates and extremely low disp...
This technical note provides an overview of the Agilent Original Bundle PC offer. Agilent bundle PCs are HP computers fully tested and qualified to run Agilent Chromatography, Mass Spectroscopy and UV-Vis Spectroscopy software. Agilent bundle PCs with Agilent instrument control software offer a o...
A new protocol was developed to automate sample preparation steps (cleansing and desalting) of peptides designated for mass spectrometry and the subsequent spotting onto a MALDI target plate using the Agilent Bravo Automated Liquid Handling Platform and the newly designed C18 filled VeloTips from...
The most important performance parameter for an ultrahigh performance liquid chromatography (UHPLC) pump is precision of retention times. Highest precision is required to enable identification of compounds by retention times and subsequently to be able to accurately quantify the analyzed sample c...
This application note describes a nontargeted, discovery-based approach for analyzing the yeast metabolome in response to calcium and immunosuppressant drug treatment using multiple LC conditions and the Agilent 6530 Accurate-Mass Q-TOF mass spectrometer. Differential analysis between control and...
A method has been developed on the Agilent 7000 GC Triple Quadrupole GC/MS/MS system for the analysis of 20 chemicals that may be present in printing inks applied to the external surface of cartonboard packaging material. These substances have the potential to diffuse through the cartonboard or t...
The Agilent Instrument Control Framework (ICF) enables other providers of LC data acquisition and processing software to simplify the development of software control for Agilent LC instruments. Dionex Chromeleon 6.80SR11 A in combination with Agilent ICF provides enhanced control functions for al...
In 2011, five members of the “synthetic cannabinoids” group or ‘Spice’ compounds were banned in the USA. The substances were: • 1-pentyl-3-(1-naphthoyl)-indole (JWH-018) • 1-butyl-3-(1-naphthoyl)-indole (JWH-073) • 1-[2-(4-morpholinyl)ethyl]-3-(1-naphthoyl)-indole (JWH-200) • 5-(1,1-dimethylhepty...
An Agilent 1290 Infinity LC System is optimized for the lowest possible extra-column volume, to ensure minimal band broadening and optimal results from a small dimension, Agilent ZORBAX RRHD Eclipse Plus C18, 2.1 × 50 mm, 1.8 μm column. Agilent Technologies Ultra-Low Dispersion Tubing, Ultra-Low ...
The stringency of QC tests have failed to keep pace with the advances achieved in column deactivation procedures. When pass rates rise and failure rates drop, it is time to design a more critical QC test. This application note describes a rigorous QC column testing approach that employs active te...
This Application Note describes a modified method to introduce large insertions in plasmids in two simple steps using the QuikChange II Site-Directed Mutagenesis kit. Dr. Wenge Wang in Dr. Wafik S. El-Deiry’s lab at the University of Pennsylvania has used the QuikChange II kit and a modification ...
Commercially available autosampler hardware from Agilent was used to upgrade an Agilent 1100 Series HPLC in order to eliminate the need for offline or online solid phase extraction. Using a 1800 μL large-volume injection (LVI) sample in conjunction with liquid chromatography and tandem mass spect...
During production and distribution of natural gas it is of high importance to determine its composition and calorific value because natural gas is bought and sold on its energy content. This application note shows the use of the Agilent 490 Micro GC Natural Gas Analyzer for the analysis of natura...
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