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One-way analysis of variance (ANOVA) tests allow you to determine if one given factor, such as drug treatment, has a significant effect on gene expression behavior across any of the groups under study. A significant p-value resulting from a 1-way ANOVA test would indicate that a gene is differentially expressed in at least one of the groups analyzed. If there are more than two groups being analyzed, however, the 1-way ANOVA does not specifically indicate which pair of groups exhibits statistical differences. Post Hoc tests can be applied in this specific situation to determine which specific pair/pairs are differentially expressed. This document will provide the necessary information for you to perform these analyses within GeneSpring.
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